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| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Chemistry | 20–25% | - Electrolytes, acid-base, and toxicology - Carbohydrates, lipids, and enzymes |
| Topic 2: Microbiology | 15–20% | - Virology and parasitology testing - Bacteriology and mycology procedures |
| Topic 3: Immunology | 5–10% | - Serologic procedures and immune diseases - Principles of immunology |
| Topic 4: Laboratory Operations | 5–10% | - Quality assessment and safety - Instrumentation and laboratory mathematics |
| Topic 5: Urinalysis and Other Body Fluids | 5–10% | - Microscopic analysis and disease correlation - Physical and chemical testing |
| Topic 6: Hematology | 20–25% | - Laboratory testing and hemostasis - Physiology and disease states |
| Topic 7: Blood Banking | 15–20% | - Blood products and components - Transfusion practice and testing - Blood group systems and immunology |
1. If your reactions are strong at immediate spin (3+) and then get weaker at AHG (w+), it could mean the presence of a strong cold antibody.
Cold antibodies tend to be IgM and their optimum phase for reactivity is immediate spin. Incubation and washing of the sample may cause the agglutination that occurred at room temperature to break down. This would appear as a weaker reaction at AHG.
If the reaction strengths varied in each panel cell then that could be an indication that there are multiple antibodies present.
Your screen cells are 3+ at immediate spin and weak (W)+ at AHG. Your auto control is negative for both phases. Some of your antibody panel cells are 3+ at immediate spin and negative at AHG. What should you suspect?
A) A warm autoantibody is present
B) Bad specimen draw
C) A cold antibody may be present
2. Gram positive organisms resist decolorization by ethyl alcohol. The large crystal violet-iodine complex is not able to penetrate the peptidoglycan layer, and is trapped within the cell in gram-positive organisms.
Conversely, the outer membrane of gram-negative organisms is degraded and the thinner peptidoglycan layer of gram-negative cells is unable to retain the crystal violet-iodine complex and the color is lost.
What is the purpose of using ethyl alcohol or acetone in the gram stain procedure:
A) Decolorize all gram positive organisms
B) Fix all gram negative organisms
C) Fix all gram positive organisms
D) Decolorize all gram negative organisms
3. Serum ferritin is a good indicator of iron deficiency. However, it acts like an acute phase reactant, being elevated in a large number of conditions. Patients who have iron deficiency as well as another condition that elevates serum ferritin levels may therefore have normal or even elevated serum ferritin levels.
Which one of the following statements about serum ferritin are true:
A) It is a sensitive indicator of iron deficiency
B) It is synonymous with transferrin
C) Elevation of serum ferritin is specific for hemochromatosis
D) It is a better test for bone marrow iron stores than a Prussian blue stain of marrow particles
4. Increased concentrations of alpha-fetoprotein (AFP) in adults are MOST characteristically associated with:
A) multiple myeloma
B) chronic active hepatitis
C) hepatocellular carcinoma
D) alcoholic cirrhosis
5. Epidemiology studies only infectious diseases that impact humans
A) True
B) False
Solutions:
| Question # 1 Answer: C | Question # 2 Answer: D | Question # 3 Answer: A | Question # 4 Answer: C | Question # 5 Answer: A |
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